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Diagnostic Testing

iSpot LymeTM: A New Generation of Lyme Disease Testing

Chenggang Jin, PhD, MDa,b, Deanna J. Fall, BAc, Diana Roen BS,a and Gottfried Kellermann, PhDc

Abstract
Lyme disease is the most reported vectorborne illness in the United States. The incidence may be vastly under-reported due to limitations of current testing methodologies; the need for more sensitive and specific tests is apparent. The iSpot LymeTM assay is a new generation of in vitro diagnostic test for the detection of antigen-specific effector/memory T cells that respond to stimulation by Borrelia burgdorferi antigens. The highly sensitive T cell-based enzyme-linked immunospot (ELISPOT) methodology enumerates the Borrelia burgdorferi activated effector/memory T cells. This assay is read more…

Evaluation of a Novel Elisa for Serotonin: Urinary Serotonin as a Potential Biomarker for Depression

Mikaela I. Nichkova & Han Huisman & Paul M. Wynveen & David T. Marc & Kelly L. Olson & Gottfried H. Kellermann

Abstract
Depression is a common disorder with physical and psychological manifestations often associated with low serotonin. Since noninvasive diagnostic tools for depression are sparse, we evaluated the clinical utility of a novel ELISA for the measurement of serotonin in urine from depressed subjects and from subjects under antidepressant therapy. We developed a competitive ELISA for direct measurement of serotonin in derivatized urine samples. Assay performance was evaluated and applied to clinical samples. The analytical range of the assay read more…

Urinary Neurotransmitter Testing: A Powerful Tool for Assessing Nervous System Function

Bradley Bush, ND

Abstract
Neurotransmitters are traditionally described as brain chemicals that act as messengers in the body, relaying signals between neurons. Neurotransmitter levels in the urine are derived from a variety of sources including the CNS, PNS and the immune system. As such, they’re incredibly powerful. Present throughout the body, neurotransmitters are required for proper brain and body functions and regulate numerous physical and emotional processes, such as mental performance, emotional states and pain response. They are responsible for mood, pain and pleasure sensations and control the quality of sleep, appetite and energy levels, and sex drive. read more…

The Clinical Utility of Urinary Neurotransmitter Analysis: An Overview

Joe Ailts,BS, Kelly Olson, Ph.D

Abstract
Neurotransmitters are recognized as the primary biochemical messengers of the central and peripheral nervous systems. Studies have demonstrated that urinary neurotransmitter measures are reflective of circulating levels as evidenced by renal neurotransmitter clearance mechanisms. Laboratory methodology for the accurate assessment of urinary neurotransmitter levels has been established. Urinary measures are not recognized as a direct reflection of central activity, however definite associations exist. The ability to measure neurotransmitters has led to the generation of scientific literature that demonstrates urinary neurotransmitter measurements have clinical value as representative biomarkers of various neurological, immunological, and endocrinological conditions. read more…

Anxiety and Depression: It All Starts With Stress

Donielle Wilson, ND, CPM

Abstract
Is stress an issue for your patients? Perhaps the real question is, when is stress not an issue? Everyone is exposed to potential stressors on a daily basis. Stress comes in the form of physical stressors, as in an injury or infection, and emotional stressors, such as loss, change, or trauma. Lack of sleep and skipping meals are also stressful due to the body response that occurs in an attempt to maintain balance. Even the perception of stress stimulates the body’s stress response.

No matter the form, recent studies show that stress leads to disruptions read more…

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