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Evaluation of a Novel Elisa for Serotonin: Urinary Serotonin as a Potential Biomarker for Depression

Mikaela I. Nichkova & Han Huisman & Paul M. Wynveen & David T. Marc & Kelly L. Olson & Gottfried H. Kellermann

Abstract
Depression is a common disorder with physical and psychological manifestations often associated with low serotonin. Since noninvasive diagnostic tools for depression are sparse, we evaluated the clinical utility of a novel ELISA for the measurement of serotonin in urine from depressed subjects and from subjects under antidepressant therapy. We developed a competitive ELISA for direct measurement of serotonin in derivatized urine samples. Assay performance was evaluated and applied to clinical samples. The analytical range of the assay read more…

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